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DIG-High Prime DNA Labeling and Detection Starter Kit II

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产品名称: DIG-High Prime DNA Labeling and Detection Starter Kit II
产品型号: 11585614910
产品展商: Roche
产品文档: 无相关文档

简单介绍

For 12 random-primed DNA labeling reactions with DIG-11-dUTP, alkali-labile, and 24 chemiluminescence detection reactions with CSPD, ready-to-use. 硕嘉生物常年现货销售roche的地高辛DIG标记检测试剂盒KitII(化学发光),欢迎广大客户来电咨询!


DIG-High Prime DNA Labeling and Detection Starter Kit II  的详细介绍

DIG-High Prime DNA Labeling and Detection Starter Kit II

roche的地高辛DIG标记检测试剂盒KitII(化学发光)

Sample Materials

·                                 DNA fragments of at least 100 bp

·                                 Linearized plasmid, cosmid or λDNA

·                                 Supercoiled DNA

Sensitivity and specificity: Standard assay uses 1 µg of linearized pBR328. It is usual to expect 0.8 µg labeled DNA after 1 hour of labeling, and 2.3 µg labeled DNA after 20 hours reaction. In a dot blot hybridization, 0.03 pg of homologous DNA is detectable with chemiluminescence (probe concentrations at 20 ng/ml). A single copy human gene (t-PA) is detectable in a Southern blot, loading 1 µg of digested placenta DNA.
 

Principle

The DIG High Prime DNA Labeling and Detection Starter Kit II uses digoxigenin (DIG), a steroid hapten, to label DNA probes for hybridization and subsequent chemiluminescence detection by enzyme immunoassay. The "random primed" DNA labeling method originally developed by Feinberg and Vogelstein is based on the hybridization of oligonucleotides of all possible sequences to the denatured DNA to be labeled. The input DNA serves solely as a template for the synthesis of labeled DNA, and is not degraded during the reaction, making it possible to label minimal amounts of DNA (10 ng) with this method.The complementary DNA strand is synthesized by Klenow polymerase using the 3'-OH termini of the random oligonucleotides as primers. Modified deoxyribonucleoside triphosphates, labeled with digoxigenin present in the reaction, are incorporated into the newly synthesized complementary DNA strand.
 

Quality

Function tested in a dot blot.

 

上海硕嘉生物常年现货销售DIG-High Prime DNA Labeling and Detection Starter Kit II,roche的地高辛DIG标记检测试剂盒KitII(化学发光),2015年全年促销该产品,欢迎广大客户来电咨询!

DIG-High Prime DNA Labeling and Detection Starter Kit II

DIG-High Prime DNA Labeling and Detection Starter Kit II

DIG-High Prime DNA Labeling and Detection Starter Kit II

 

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